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Related Experiment Video

Updated: May 6, 2026

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

Random-amplified-polymorphic DNA markers in sorghum.

S Pammi¹, K Schertz, G Xu

¹Department of Biochemistry and Biophysics, 3USDA-ARS Texas A&M University, 77843, College Station, TX, USA.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik

|November 2, 2013

Summary

This summary is machine-generated.

Reproducible amplification of Random Amplified Polymorphic DNA (RAPD) markers in sorghum was achieved. Optimized conditions and radiolabeling enabled high-resolution RAPD analysis, revealing genetic polymorphisms crucial for sorghum breeding.

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Published on: March 4, 2021

Area of Science:

Plant genetics
Molecular biology
Biotechnology

Background:

Random Amplified Polymorphic DNA (RAPD) markers are valuable for genetic studies.
Reproducible RAPD marker amplification in sorghum requires optimized reaction conditions.
High-resolution analysis of RAPD markers is essential for accurate genetic mapping.

Purpose of the Study:

To establish reproducible amplification conditions for RAPD markers in sorghum.
To develop a high-resolution method for RAPD marker analysis.
To assess the utility of RAPD markers for genetic mapping in sorghum.

Main Methods:

Optimized Polymerase Chain Reaction (PCR) parameters, including MgCl2 concentration and temperature.
Radiolabeling of PCR-amplified DNA followed by separation on denaturing polyacrylamide gels.
Application of RAPD markers across 32 sorghum genotypes and mapping in an F2 population.

Main Results:

Identified specific conditions for reproducible RAPD marker amplification in sorghum.
Achieved high-resolution RAPD analysis through radiolabeling and gel electrophoresis.
Approximately 80% of tested primers amplified DNA, revealing 1-5 polymorphisms between sorghum lines.
Successfully mapped RAPD markers using an F2 population, correlating with an existing RFLP map.

Conclusions:

Optimized conditions ensure reproducible RAPD marker amplification in sorghum.
High-resolution RAPD analysis is feasible and effective for detecting genetic variation.
RAPD markers are a valuable tool for genetic mapping and marker-assisted selection in sorghum breeding programs.