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Updated: May 6, 2026

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Practical considerations for maintaining germplasm in maize.

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  • 1International Maize and Wheat Improvement Center (CIMMYT), Lisboa 27, Apdo. Postal 6-641, Mexico, D.F., Mexico.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|November 2, 2013
PubMed
Summary
This summary is machine-generated.

Effective maize (Zea mays L.) regeneration conserves genetic diversity by controlling male and female gametes. Stratified sampling aids in selecting core subsets for maize collections.

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Area of Science:

  • Agriculture
  • Genetics
  • Plant Science

Background:

  • Genetic resource management is crucial for crop and wild relative diversity.
  • Ex-situ conservation strategies are vital for maintaining valuable genetic traits.

Purpose of the Study:

  • To review practical regeneration methods for maize (Zea mays L.) based on population genetics.
  • To describe challenges in selecting core subsets from maize germplasm collections.

Main Methods:

  • Controlled hand pollination to manage male gametes (pollen parents).
  • Harvesting equal numbers of kernels from each seed plant to manage female gametes.
  • Stratified sampling procedures for delineating core subsets from racial collections.

Main Results:

  • Controlled regeneration doubles the effective population size (Ne) compared to the original population (N).
  • Practical examples of controlling parent gametes during regeneration are provided.
  • A core subset delineation from a Tuxpeño racial collection is illustrated.

Conclusions:

  • Controlled regeneration methods are essential for maintaining genetic diversity in ex-situ maize conservation.
  • Stratified sampling offers a systematic approach to forming representative core subsets of maize germplasm.