Development a novel approach of chemiluminescent probe array

Area of Science:

• Materials Science
• Analytical Chemistry
• Biotechnology

Background:

• Developing advanced materials for sensitive analytical assays is crucial.
• Mesoporous nanocomposites offer unique properties for catalysis and immobilization.
• Existing solid-phase supports have limitations in probe array development.

Purpose of the Study:

• To develop a novel bifunctional chemiluminescent (CL) probe array.
• To utilize a Co3O4-SiO2 mesoporous nanocomposite material.
• To apply the assay for high-throughput glucose determination.

Main Methods:

• Fabrication of a Co3O4-SiO2 mesoporous nanocomposite.
• Immobilization of enzymes onto the nanocomposite material.
• Development of a luminol-H2O2 CL reaction for detection.
• Application in a probe array for glucose quantification.

Main Results:

• The Co3O4-SiO2 nanocomposite exhibited excellent catalytic activity for the CL reaction.
• The bifunctional probe array demonstrated efficient enzyme immobilization.
• Successful high-throughput determination of glucose in human samples.
• Achieved a linear range of 3-90 μM and a detection limit of 0.36 μM for glucose.

Conclusions:

• The developed CL probe array assay offers a new approach for sensitive analyte detection.
• Mesoporous nanocomposites provide a versatile platform for probe development.
• This work offers new insights into solid-phase support applications in analytical chemistry.