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Updated: May 6, 2026

Embryo Rescue Protocol for Interspecific Hybridization in Squash
Published on: September 12, 2022
In situ gynogenetic haploid plants of chicory (Cichorium intybus L.) after intergeneric hybridization with Cicerbita
1Station de Génétique et d'Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint-Cyr, F-78026, Versailles Cedex, France.
Abstract:
The possibility of obtaining haploid plants of chicory (Cichorium intybus L.) was investigated through intergeneric hybridization. Chicory plants (industrial chicory and Chioggia) were pollinated with pollen of Lactuca tatarica L. and Cicerbita alpina Walbr. Many achenes contained embryos which were rescued in vitro. Only a few embryos developed into plants which were then acclimatized in soil. Among them, three expressed a chicory phenorype and were haploid.
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