Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

9.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hybrid capture RNA-seq defines temporal gene expression in <i>Rickettsia</i>.

mSphere·2026
Same author

Hybrid capture RNA-seq defines temporal gene expression in <i>Rickettsia</i>.

bioRxiv : the preprint server for biology·2026
Same author

An expanded genetic toolkit for inducible expression and targeted gene silencing in <i>Rickettsia parkeri</i>.

Journal of bacteriology·2024
Same author

An expanded genetic toolkit for inducible expression and targeted gene silencing in <i>Rickettsia parkeri</i>.

bioRxiv : the preprint server for biology·2024
Same author

An evolutionarily conserved stop codon enrichment at the 5' ends of mammalian piRNAs.

Nature communications·2022
Same author

Viral recombination systems limit CRISPR-Cas targeting through the generation of escape mutations.

Cell host & microbe·2021
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 6, 2026

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

11.4K

Small RNA library construction for high-throughput sequencing.

Jon McGinn1, Benjamin Czech

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 2, 2013
PubMed
Summary
This summary is machine-generated.

High-throughput sequencing advances the study of small RNAs, crucial regulatory molecules in biological processes. This protocol details small RNA library preparation for Solexa/Illumina sequencing, enhancing reproductive tissue research.

More Related Videos

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
08:37

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Published on: October 9, 2020

6.0K
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.6K

Related Experiment Videos

Last Updated: May 6, 2026

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

11.4K
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
08:37

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Published on: October 9, 2020

6.0K
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.6K

Area of Science:

  • Molecular Biology
  • Genomics
  • Reproductive Biology

Background:

  • Small RNAs are critical regulatory molecules discovered ~20 years ago.
  • High-throughput sequencing has significantly advanced understanding of small RNA functions.
  • Small RNAs play vital roles in reproductive tissues, requiring high-resolution analysis.

Purpose of the Study:

  • To describe a protocol for preparing small RNA libraries for sequencing.
  • To enable high-resolution analysis of small RNAs in reproductive tissues using Solexa/Illumina technology.

Main Methods:

  • Protocol development for small RNA library preparation.
  • Application of Solexa/Illumina sequencing technology.
  • Analysis of millions of individual small RNA sequences.

Main Results:

  • A detailed protocol for small RNA library preparation is presented.
  • The protocol is optimized for Solexa/Illumina sequencing platforms.
  • The method provides high resolution necessary for studying small RNAs in reproductive tissues.

Conclusions:

  • The described protocol facilitates the study of small RNAs using high-throughput sequencing.
  • This method enhances the understanding of small RNA roles in reproductive biology.
  • The protocol offers a valuable tool for researchers in molecular and reproductive sciences.