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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Updated: May 6, 2026

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

Proteomics methods to study methionine oxidation.

Bart Ghesquière¹, Kris Gevaert

¹Department of Medical Protein Research, VIB, B-9000, Ghent, Belgium; Department of Biochemistry, Ghent University, B-9000, Ghent, Belgium.

Mass Spectrometry Reviews

|November 2, 2013

Summary

This summary is machine-generated.

Oxidative stress impacts protein function and signaling via methionine oxidation. New mass spectrometry and proteomics methods help characterize this crucial modification, advancing our understanding of its cellular roles.

Keywords:

PTM analysis methionine oxidation methionine sulfoxide oxidative stress redox proteomics

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Area of Science:

Biochemistry and Molecular Biology
Cellular Signaling
Oxidative Stress Research

Background:

Protein-bound methionine oxidation is key to understanding oxidative stress effects on protein function and cellular signaling.
Limited technologies and specific antibodies hinder the study of methionine sulfoxides and their proteome-wide impact.
Enzyme repair models highlight the significant cellular role of methionine oxidation.

Purpose of the Study:

To review existing mass spectrometry-based and proteomics methods for characterizing in vivo methionine oxidation.
To address the technological gaps in studying methionine sulfoxides.
To underscore the importance of methionine oxidation in cellular processes.

Main Methods:

Review of mass spectrometry-based proteomics techniques.
Analysis of methodologies for in vivo methionine oxidation characterization.
Discussion of challenges in antibody development for methionine sulfoxides.

Main Results:

Identification of various mass spectrometry and proteomics approaches for studying methionine oxidation.
Highlighting the limitations of current technologies for comprehensive analysis.
Emphasizing the necessity for advanced methods to understand methionine oxidation's role.

Conclusions:

Mass spectrometry and proteomics offer powerful tools for investigating methionine oxidation in vivo.
Further technological development is crucial for a complete understanding of methionine oxidation's cellular significance.
Characterizing methionine oxidation is vital for deciphering cellular responses to oxidative stress.