Changes in voltage-dependent calcium currents during the cell cycle of the ciliate Stylonychia

Electrophysiological properties of the hypotrichous ciliate Stylonychia mytilus were studied at two stages of its cell cycle: within 30 min after cell division and several hours thereafter. The action potential wave form, and the relative amount of two voltage-dependent calcium inward currents are significantly different in 'young' daughter cells as compared with 'adult' cells. The ratio between total inward and outward current is also larger in 'young' cells. The results provide evidence that during its cell cycle Stylonychia undergoes qualitative developmental changes with respect to its ionic channels in the membrane. These changes may explain the different cell behaviour observed up to 1 h after cell division.