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Updated: May 6, 2026
Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro
Published on: December 26, 2014
Efficacy and side effects of granulocyte collection in healthy donors
Franziska Brockmann1, Michael Kramer, Martin Bornhäuser
1Department of Anesthesiology, University Hospital, Tübingen, TU-Dresden, Germany.
Background:
We report on the efficacy and side effects of granulocyte collection, which is comparatively infrequently performed in Germany.
Methods:
Data from 378 healthy donors who underwent 914 granulocyte collections between 1999 and 2007 were retrospectively analyzed. Donors received G-CSF (lenograstim) at a median dose of 5.58 (3.25-7.36) μg/kg body weight with (n = 243) or without (n = 57) 4 mg dexamethasone. Side effects were recorded by donor monitoring and interview (questionnaire).
Results:
The median granulocyte yield in apheresis products was 8.47 × 10(10) (3.07-14.92 × 10(10)). Granulocyte yields correlated significantly with gender, baseline WBC, PMN and PLT counts, and nicotine consumption. Dexamethasone and lenograstim administration was more effective than lenograstim administration alone (p < 0.001). Side effects of granulocyte mobilization were generally mild: bone pain in 31.4%, headache in 19.6%, and fatigue in 15.7% of donors. During follow-up (4 weeks), pruritus and/or exanthema were reported in 17.6% of donors.
Conclusions:
Granulocyte mobilization with lenograstim with or without dexamethasone was a safe and effective regimen for granulocyte mobilization. Side effects were tolerable and milder than those seen in peripheral blood stem cell donors. Long-term monitoring of granulocyte donors is important to establish optimal standards for the procedure.
