Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ascidians as a Sustainable Source of Cellulose: Physicochemical Characterization, Degradability, and Relevance for Bioplastic Applications.

Biopolymers·2026
Same author

Advances in β-titanium alloys for safer and greener biomedical implants.

Biomaterials advances·2026
Same author

Preclinical testing of acellular and polyphenol-stabilized arterial xenografts in sheep.

The journal of vascular access·2025
Same author

The Evaluation of Cellulose from Agricultural Waste as a Polymer for the Controlled Release of Ibuprofen Through the Formulation of Multilayer Tablets.

Bioengineering (Basel, Switzerland)·2025
Same author

Exploiting Spiropyran Solvatochromism for Heavy Metal Ion Detection in Aqueous Solutions.

ACS omega·2025
Same author

Polyacrylonitrile/Silver Nanoparticles Composite for Catalytic Dye Reduction and Real-Time Monitoring.

Polymers·2025

Related Experiment Video

Updated: May 6, 2026

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device
07:05

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Published on: April 4, 2018

13.7K

Janus magnetic cellular spheroids for vascular tissue engineering.

Brandon M Mattix1, Timothy R Olsen, Megan Casco

  • 1Department of Bioengineering, Clemson University, 301 Rhodes Research Center, Clemson, SC 29634, USA.

Biomaterials
|November 5, 2013
PubMed
Summary

This study introduces Janus magnetic cellular spheroids (JMCS) that spatially separate cells and magnetic nanoparticles (MNPs). This novel approach minimizes MNP internalization, preserving cell viability and enabling precise magnetic manipulation for tissue engineering.

Keywords:
Iron oxideMagnetic nanoparticlesSpheroidsTissue engineeringTissue fusion

More Related Videos

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
09:46

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

Published on: April 27, 2017

9.7K
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

20.8K

Related Experiment Videos

Last Updated: May 6, 2026

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device
07:05

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Published on: April 4, 2018

13.7K
3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
09:46

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

Published on: April 27, 2017

9.7K
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

20.8K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Cell aggregates (spheroids) mimic native tissues for regenerative medicine and drug testing.
  • Magnetic nanoparticles (MNPs) enable spheroid manipulation but often cause adverse cellular effects due to uptake.
  • Existing MNP incorporation methods negatively impact cell activity, viability, and phenotype.

Purpose of the Study:

  • To develop a novel method for incorporating MNPs into cellular spheroids without adverse cellular effects.
  • To create Janus magnetic cellular spheroids (JMCS) with spatially segregated cell and MNP domains.
  • To demonstrate the utility of JMCS for magnetic manipulation and tissue fabrication.

Main Methods:

  • Fabrication of Janus magnetic cellular spheroids (JMCS) with distinct cell and extracellular MNP domains.
  • Quantification of MNP internalization in JMCS compared to traditional uptake methods.
  • Assessment of cellular viability in JMCS over seven weeks.
  • Magnetic force assembly of JMCS into patterned structures and tissue constructs.

Main Results:

  • JMCS significantly reduced MNP internalization (35%) compared to uptake spheroids (83%).
  • JMCS maintained high cellular viability (≥82% over 7 weeks) compared to controls.
  • JMCS demonstrated effective magnetic manipulation and fusion into complex tissue patterns.
  • A 5 mm vascular tissue construct was successfully assembled using JMCS.

Conclusions:

  • The Janus structure effectively separates cells and MNPs, preventing adverse effects.
  • JMCS offer a safe and effective platform for MNP-guided spheroid manipulation and tissue engineering.
  • This method facilitates the creation of complex, functional tissue constructs for various applications.