Related Experiment Video
Updated: May 6, 2026
Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens
Published on: January 21, 2019
Determining the concentrations of the squamous cell carcinoma antigen using the Maglumi 2000 automatic analyzer
1Shenzhen Second People's hospital, (The First Affiliated Hospital of Shenzhen University), Shenzhen, Guangdong, P.R. China - qisuwen2011@yahoo.cn.
Aim:
The aim of this paper was to validate the use of the chemiluminescence immunoassay (CLIA) for detecting the squamous cell carcinoma antigen (SCCa) in human serum or plasma using a Maglumi 2000 automatic analyzer.
Methods:
We performed a pilot study to examine both the sensitivity and specificity of the SCCa, in diagnosing squamous cell carcinomas. High levels of SCCa are helpful in diagnosing lung cancer, especially squamous cell carcinoma.
Results:
The method was linear to 22.81 ng/mL SCCa, with a detection limit of 0.02 ng/mL. An entire assay can be completed in 40 mins. The coefficients of variation (CV) of the intra- and inter-assays were less than 5% and 6%, respectively. There was a good correlation between the present and manual method. The SCCa discriminated between the squamous cell carcinoma patients and healthy controls with an area under the receiver operating characteristic (ROC) curve of 0.9231.
Conclusion:
These results indicate that the SCCa assay showed good performance using a Maglumi 2000 automatic analyzer. This new and simple analytic system will promote the application of SCCa in clinical laboratories.
More Related Videos
10:29Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
Published on: August 14, 2019
15:17Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
Published on: September 25, 2011