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Published on: November 15, 2013
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1Landwirtschaftliche Versuchsstation, BASF, 6703, Limburgerhof, Pfalz.
Bulletin of Environmental Contamination and Toxicology
|November 5, 2013
Summary
Microorganisms in soil can efficiently dephenylate the herbicide pyrazon (5-amino-4-chloro-2-phenyl-3(2H)-pyridazinone), producing a pure radioactive metabolite. This microbial process offers a cost-effective synthesis route for the labeled compound.
Area of Science:
- Environmental chemistry
- Microbiology
- Biochemical synthesis
Context:
- The herbicide pyrazon (5-amino-4-chloro-2-phenyl-3(2H)-pyridazinone) is degraded in soil.
- Microbial communities in soil can be conditioned to enhance pyrazon degradation.
- Chemical synthesis of the radiolabeled metabolite is challenging and expensive.
Purpose:
- To synthesize 5-amino-4-chloro-3(2H)-pyridazinone-(4,5-(14)C) using microbiological dephenylation.
- To establish a cost-effective method for producing the radiolabeled metabolite.
- To investigate the microbial degradation pathway of pyrazon in conditioned soil.
Summary:
- Soil microorganisms were utilized to dephenylate pyrazon-(4,5-(14)C), yielding 5-amino-4-chloro-3(2H)-pyridazinone-(4,5-(14)C).
- Conditioned soils demonstrated high degradation capacity, metabolizing up to 5000 ppm pyrazon within 14 days.
- The microbiological synthesis resulted in a 53.1% recovery of the pure, radiolabeled metabolite.
Impact:
- Provides a viable and economical method for synthesizing radiolabeled pyrazon metabolites.
- Highlights the potential of tailored microbial consortia for bioremediation and synthesis.
- Facilitates further research into the environmental fate and metabolism of pyrazon.
