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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
Published on: March 5, 2017
RFLP mapping of partially sequenced leaf cDNA clones in maize
S Chao1, C Baysdorfer, O Heredia-Diaz
1Department of Agronomy, Curtis Hall, University of Missouri, 65211, Columbi, MO, USA.
Abstract:
We report here the results of mapping a set of 92 leaf cDNA clones in maize. The ends of each of these cDNA clones have previously been partially sequenced, and the sequence comparison has revealed the putative function for 28 clones. It is expected that the RFLP map developed using these expressed sequence tags will be of great importance for future maize genome analysis, such as for PCR-based gene mapping or gene function identification.
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