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Related Concept Videos

Light Acquisition02:16

Light Acquisition

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In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
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RACE - Rapid Amplification of cDNA Ends02:35

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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Related Experiment Video

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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

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RFLP mapping of partially sequenced leaf cDNA clones in maize.

S Chao1, C Baysdorfer, O Heredia-Diaz

  • 1Department of Agronomy, Curtis Hall, University of Missouri, 65211, Columbi, MO, USA.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|November 5, 2013
PubMed
Summary
This summary is machine-generated.

Researchers mapped 92 maize leaf complementary DNA (cDNA) clones, identifying the functions of 28. This expressed sequence tag map aids future maize genome analysis and gene discovery.

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Area of Science:

  • Plant genetics
  • Molecular biology
  • Genomics

Background:

  • Maize (Zea mays) is a vital crop requiring advanced genomic tools.
  • Understanding gene function is crucial for crop improvement.

Purpose of the Study:

  • To construct a reference map of 92 leaf complementary DNA (cDNA) clones in maize.
  • To identify the putative functions of these mapped clones.
  • To facilitate future maize genome research.

Main Methods:

  • Partial sequencing of expressed sequence tag (EST) clones.
  • Sequence comparison for functional prediction.
  • Development of a restriction fragment length polymorphism (RFLP) map.

Main Results:

  • Successfully mapped 92 leaf cDNA clones in the maize genome.
  • Identified the putative functions for 28 of the mapped clones.
  • Generated a valuable RFLP map resource.

Conclusions:

  • The developed RFLP map serves as a crucial resource for maize genome analysis.
  • This map will support PCR-based gene mapping and gene function identification.
  • The findings advance the understanding of the maize transcriptome.