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Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
Published on: September 20, 2016
A high-frequency transformation system for the yeast Kluyveromyces lactis
1Institut für Mikrobiologie, Universität Düsseldorf, Universitätsstraße 1, 4000, Düsseldorf, Germany.
Abstract:
This communication describes conditions for Kluyveromyces lactis protoplast regeneration and transformation and shows that a high frequency of transformation can be obtained with recombinant plasmids containing one of a series of K. lactis DNA fragments that presumably carry autonomously replicating sequences (called KARS). The vector YRp7, which contains a Saccharomyces cerevisiae autonomously replicating sequence (ARS), only led to integrative transformation of K. lactis indicating substantial differences in specificity between the DNA replication mechanisms of both yeast species. It is further shown that 2-μm DNA derived vectors giving high frequency transformation in S. cerevisiae can transform K. lactis only with low frequency.
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