A comparison of several published methods for barley anther culture

A number of methods have been published for barley (Hordeum vulgare L.) anther culture and have gained acceptance in different laboratories. The breeder's requirement is for a compromise method that gives good, repeatable results for a wide range of genotypes. Yet the routine production of spontaneously doubled haploid green regenerants remains difficult. Despite attempts to formulate a widely-applicable anther culture method, the 4 main published methods, compared here with one modified procedure, are quite distinct for a number of important characteristics. The methods interacted strongly with the 3 genotypes, and response ranged from zero to 28 green regenerants per 100 anthers plated. The current methods still require often substantial modification to suit local situations in order that the technology may be exploited by barley breeders.