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Updated: May 6, 2026

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

Microfluidic multi-input reactor for biocatalytic synthesis using transketolase.

James Lawrence¹, Brian O'Sullivan, Gary J Lye

¹Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.

Journal of Molecular Catalysis. B, Enzymatic

|November 5, 2013

Summary

This summary is machine-generated.

A novel multi-input microfluidic reactor enables continuous-flow biocatalysis by mimicking fed-batch strategies. This approach significantly enhances output concentration and throughput for specialty chemical production.

Keywords:

Fed-batch Laser fabrication Micro reactor Microfluidic reactor Transketolase

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Area of Science:

Biocatalysis and biochemical engineering
Microfluidics and continuous-flow chemistry
Specialty chemical synthesis

Background:

Biocatalytic synthesis in continuous-flow microreactors is crucial for specialty chemicals.
High substrate concentrations can inhibit or denature biocatalysts, limiting yields.
Existing fed-batch reactors solve this but lack a continuous-flow solution.

Purpose of the Study:

To design a novel multi-input microfluidic reactor for continuous-flow biocatalysis.
To adapt fed-batch substrate feeding strategies to a continuous-flow microreactor.
To overcome limitations of high substrate concentrations in microreactor systems.

Main Methods:

Development of a multi-input microfluidic reactor for controlled substrate feeding.
Application of the transketolase (TK)-catalyzed reaction using lithium hydroxypyruvate (HPA) and glycolaldehyde (GA).
Demonstration of a transposed fed-batch substrate feeding strategy in the microreactor.

Main Results:

Achieved a 4.5-fold increase in output concentration compared to single-input reactors.
Demonstrated a 5-fold increase in throughput using the novel reactor design.
Successfully implemented a continuous-flow fed-batch strategy for biocatalysis.

Conclusions:

The multi-input microfluidic reactor effectively addresses substrate inhibition in continuous-flow biocatalysis.
This technology offers a viable continuous-flow alternative to fed-batch systems for specialty chemical production.
The developed reactor enhances both concentration and throughput, improving process efficiency.