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Neonatal hyperoxic exposure persistently alters lung secretoglobins and annexin A1

Thomas M Raffay1, Morgan L Locy, Cynthia L Hill

  • 1Nationwide Children's Hospital, The Ohio State University College of Medicine, Columbus, OH 43205, USA.

Biomed Research International
|November 5, 2013
PubMed

Insights

Neonatal hyperoxia decreases Clara cell secretory protein (CCSP) in mice, altering lung immune responses. This suggests CCSP

Area of Science:

  • Pulmonary Medicine
  • Neonatal Research
  • Immunology

Background:

  • Bronchopulmonary dysplasia (BPD) in infants involves altered lung epithelial function.
  • Decreased secretoglobins (SCGBs), including Clara cell secretory protein (CCSP), are observed in BPD.
  • CCSP influences innate immune responses, as suggested by studies in CCSP knockout mice.

Purpose of the Study:

  • To investigate if neonatal hyperoxia causes deficits in CCSP expression.
  • To determine if these deficits are linked to persistent changes in lung SCGB and annexin A1 (ANXA1) expression.
  • To explore the impact of hyperoxia-induced CCSP alterations on lung innate immune function.

Main Methods:

  • Newborn C3H/HeN mice were exposed to room air or 85% oxygen.
  • Mice were sacrificed at 14 days or recovered in room air for 14 days.
  • Lung CCSP, SCGB3A1 protein/mRNA, ANXA1 charge characteristics, and macrophage numbers were analyzed.

Main Results:

  • Neonatal hyperoxia followed by room air recovery decreased lung CCSP and SCGB3A1 protein, but not mRNA.
  • Hyperoxia-induced alterations in ANXA1 charge persisted after room air recovery.
  • These changes were associated with increased lung macrophage numbers.

Conclusions:

  • Neonatal hyperoxia impairs CCSP expression, affecting lung SCGBs and ANXA1.
  • Altered Clara cell function impacts lung innate immunity via immunomodulatory proteins.
  • Further research is needed to elucidate CCSP's role in BPD pathogenesis.

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