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Updated: May 6, 2026

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
Published on: January 11, 2019
Electron paramagnetic resonance resolves effects of oxidative stress on muscle proteins
Rebecca J Moen1, Jennifer C Klein, David D Thomas
11Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN; 2Department of Chemistry and Geology, Minnesota State University, Mankato, Mankato, MN; and 3Department of Biology, University of Wisconsin, Lacrosse, Lacrosse, WI.
Abstract:
We have used site-directed spin labeling and electron paramagnetic resonance (EPR) to explore the effects of oxidation on muscle function, with particular focus on the actin-myosin interaction. EPR measurements show that aging or oxidative modification causes a decrease in the fraction of myosins in the strong-binding state, which can be traced to the actin-binding cleft of the myosin catalytic domain.
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