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Updated: May 6, 2026
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
Published on: June 18, 2020
Probing subcellular organic hydroperoxide formation via a genetically encoded ratiometric and reversible fluorescent
Boxuan Simen Zhao1, Gong Zhang, Shizhe Zeng
1Synthetic and Functional Biomolecules Center (SFBC), Beijing National Laboratory for Molecular Sciences (BNLMS), Department of Chemical Biology, College of Chemistry, Peking University, Beijing, 100871, China. pengchen@pku.edu.cn.
Abstract:
A ratiometric and reversible organic hydroperoxide (OHP) sensor, rOHSer, was developed with high sensitivity and selectivity for subcellular OHP visualization. Through targeting rOHSer to the nucleus, we demonstrated that high levels of D-glucose cause elevated OHP production in this compartment. Further utilization of rOHSer probe may allow more elucidation of unique roles of OHPs in diverse biological processes.
