Somatic embryogenesis and plant regeneration of Gladiolus (Gladiolus hort.)
1Department of General Botany, Faculty of Biology, University of Poznań, 61-713, Poznań, Poland.
Abstract:
Friable embryogenic callus and somatic embryos of 4 Gladiolus cultivars were obtained on Murashige and Skoog (MS) medium with various concentration of auxins from the following explants: corm slices, young leaf bases and whole, intact plantlets. Somatic embryos transferred on MS hormone-free medium regenerated into plantlets. All plantlets obtained through embryogenesis did not differ phenotypically from the parental clones. The embryogenic friable callus has been maintained for over 2 years in culture and has retained a very high regeneration capacity.
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