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Published on: January 4, 2017
[Micro-CT imaging research of acoustic capsule in mice]
Cheng-cheng Sun1, Zi-dong Jiang, Kai Zhang
1Department of Otorhinolaryngology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
Objective:
To get the three-Dimensional imaging model of internal ear and middle ear using the Micro-CT equipment, and research the nondestructive imaging of mice acoustic capsule, and investigate the application of Micro-CT inmorphology of acoustic capsule.
Methods:
The mice were executed to harvest the acoustic capsules. The acoustic capsules were fixed in trinitrophenol before scanning by Micro-CT .Using this equipment, we obtained a 3D model of reconstruction from 2D images.
Results:
The 3D model of acoustic capsule and the model was extraordinary clear to show the eardrum, ossicles chain, vestibulum, semicircular canal, and cochlear. The 3D structure model was excellent for look through, and it was free to revolve in any axial direction on the platform of software Amira-5.4.
Conclusions:
By using Micro-CT equipment to research the nondestructive three-Dimensional imaging of acoustic capsule, the outside structures and internal details of the acoustic capsule can be observed at the same time. 3D structure model which was good for looking through. Using Micro-CT equipment is easy to operate and also can save a lot of samples, and the images have high resolution ratio. Micro-CT has good prospects for development.
