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Stimulation of root development on buckwheat thin cell-layer explants by pectic fragments from pea stem cell walls
V V Lozovaya1, O A Zabotina, N I Rumyantseva
1Institute of Biology of the Russian Academy of Sciences, P.O. Box 30, 420503, Kazan, Russia.
Plant Cell Reports
|November 8, 2013
Summary
Pectic fragments from pea stems significantly enhanced buckwheat root formation and growth. This study proposes a novel, sensitive buckwheat thin cell-layer bioassay for oligosaccharins.
Area of Science:
- Plant biology
- Biochemistry
- Cell biology
Background:
- Oligosaccharins are plant signaling molecules derived from cell wall fragments.
- Pectic polysaccharides are key components of plant cell walls with potential biological activities.
- Plant tissue culture systems are valuable for studying plant growth regulators.
Purpose of the Study:
- To investigate the root-inducing activity of specific pectic fragments on buckwheat.
- To evaluate the potential of buckwheat thin cell-layers (TCLs) as a bioassay for oligosaccharins.
Main Methods:
- Buckwheat (Fagopyrum esculentum Moench.) TCLs were cultured individually in a liquid medium.
- Pectic fragments (degree of polymerization 20-25) isolated from pea (Pisum sativum L.) stem cell walls were applied.
- Root formation and growth (fresh and dry weight, root number) were compared to untreated controls.
Main Results:
- Pectic fragments significantly accelerated root formation on buckwheat explants compared to controls.
- Treatment with pectic fragments promoted root growth, evidenced by increased fresh and dry weights.
- The number of roots formed was approximately doubled in treated explants.
Conclusions:
- Pectic fragments isolated from pea stems exhibit potent root-inducing activity.
- The buckwheat TCL system demonstrates high sensitivity, reproducibility, and ease of preparation, making it a suitable bioassay for oligosaccharins.
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