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Updated: May 6, 2026

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat Fagopyrum tataricum
Published on: March 11, 2020
Stimulation of root development on buckwheat thin cell-layer explants by pectic fragments from pea stem cell walls
V V Lozovaya1, O A Zabotina, N I Rumyantseva
1Institute of Biology of the Russian Academy of Sciences, P.O. Box 30, 420503, Kazan, Russia.
Abstract:
Buckwheat (Fagopyrum esculentum Moench.) thin cell-layers (TCLs) cultured individually in a liquid medium were used to test the root-inducing activity of pectic polysaccharides with a degree of polymerization (DP) of 20-25, isolated from pea (Pisum sativum L.) stem cell walls. These pectic fragments induced more rapid root formation on the explants in comparison with untreated controls. This pectic fragment treatment also promoted root growth as measured by both fresh and dry weights and about doubled the number of roots formed. This buckwheat TCL system is proposed as a new bioassay for oligosaccharins due to its sensitivity, reproducibility and ease of preparation.
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