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Investigating the reactivities of a polyketide synthase module through fluorescent click chemistry
Amanda Jane Hughes1, Matthew R Tibby, Drew T Wagner
1Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, TX, USA. adriankc@utexas.edu.
Chemical Communications (Cambridge, England)
|November 8, 2013
Abstract:
A method for monitoring in vitro polyketide synthesis has been developed whereby nonchromophoric polyketide products are made brightly fluorescent in a simple, rapid, inexpensive, and bioorthogonal manner through CuAAC with a sulforhodamine B azide derivative.
