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Humic substances shorten human plasma prothrombin time.

F J Lu1, W W Lin, T S Huang

  • 1Departments of Biochemistry, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China.

Environmental Geochemistry and Health
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Summary
This summary is machine-generated.

Humic substances and lignin shorten prothrombin time in human plasma. This effect, unlike that of monomeric compounds, was prevented by reducing agents, suggesting polymer structures impact blood coagulation enzymes.

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Area of Science:

  • Biochemistry
  • Environmental Science

Background:

  • Prothrombin time (PT) is a critical measure of blood coagulation.
  • Humic substances and lignin are complex organic polymers found in natural environments.

Purpose of the Study:

  • To investigate the effect of humic substances and lignin on prothrombin time.
  • To determine if monomeric components of humic acid share this effect.
  • To explore the role of reducing agents in modulating these effects.

Main Methods:

  • Human pooled plasma was incubated with varying concentrations of humic acid, well water humic substances, and lignin.
  • Prothrombin time was measured to assess coagulation.
  • Monomeric phenolic compounds and various reducing agents were tested for their effects.

Main Results:

  • Humic acid, well water humic substances, and lignin significantly shortened prothrombin time.
  • Monomeric components of humic acid did not affect prothrombin time.
  • Reducing agents like ascorbic acid and dithiothreitol prevented the prothrombin time shortening.

Conclusions:

  • Polymerized humic substances and lignin possess the ability to influence blood coagulation enzyme activity.
  • The observed effect is specific to the polymeric structure, not individual phenolic monomers.
  • Reducing conditions can counteract the procoagulant effects of these natural polymers.