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Updated: May 6, 2026

Scalable Transfection of Maize Mesophyll Protoplasts
Published on: June 23, 2023
A protoplast to plant system for the chrysanthemum Dendranthema zawadskii x D. grandiflora
1MAF Technology, Ministry of Agriculture and Fisheries, Horticultural Research Centre, Private Bag, Levin, New Zealand.
Abstract:
Plantlets were regenerated from protoplasts of in vitro shoot cultures and leaf-derived de novo shoots of the chrysanthemum Dendranthema zawadskii x D. grandiflora. Isolated protoplasts reformed cell walls and then began to divide within 24 hours of culture in streaky plate agarose lenses surrounded by liquid V-KM medium. Twenty one days after isolation, 1 mm diameter callus clumps were transferred to shoot regeneration medium. After a further 33 days leaves became visible. Elongated shoots were rooted on half strength hormone-free MS medium.
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