Related Experiment Video
Updated: Jan 29, 2026
08:49
Floral-Dip Transformation of Flax Linum usitatissimum to Generate Transgenic Progenies with a High Transformation Rate
Published on: December 19, 2014
29.9K
Transformation of lisianthus (Eustoma grandiflorum)
S E Ledger1, S C Deroles1, D G Manson1
1Levin Research Centre, Crop and Food Research, Private Bag 4005, Levin, New Zealand Fax no.: 64-6-368-3578; E-mail: Deroless@crop.cri.nz, , , , , , NZ.
Plant Cell Reports
|February 8, 2019
Summary
Transformed lisianthus plants were created using Agrobacterium tumefaciens. Genetic analysis confirmed successful gene integration and expression, demonstrating a viable method for plant genetic modification.
Area of Science:
- Plant biotechnology
- Molecular biology
- Genetics
Background:
- Eustoma grandiflorum (lisianthus) is a commercially important ornamental plant.
- Genetic transformation provides a powerful tool for crop improvement and research.
Purpose of the Study:
- To develop a method for the genetic transformation of Eustoma grandiflorum (lisianthus).
- To confirm the integration and expression of transgenes in transformed lisianthus plants.
Main Methods:
- Co-cultivation of young leaf pieces with Agrobacterium tumefaciens strain A722.
- Use of binary vectors pKIWI110 (containing β-D-glucuronidase reporter gene) and pLN26 (containing chalcone synthase antisense gene).
- Selection of transformants using the neomycin phosphotransferase II gene and Southern DNA analysis.
Main Results:
- Successfully produced transformed lisianthus plants from three cultivars.
- Southern DNA analysis confirmed transgene integration into the plant genome for all tested transformants.
- Plants transformed with pKIWI110 exhibited β-D-glucuronidase expression and passed the kanamycin-resistant trait to the F1 generation.
Conclusions:
- Agrobacterium-mediated transformation is effective for Eustoma grandiflorum.
- Transgenes can be stably integrated and expressed in lisianthus, with potential for heritability.
Related Concept Videos
01:33Bacterial Transformation
59.8K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
59.8K
01:26Transformers
1.8K
A device that transforms voltages from one value to another using induction is called a transformer. A transformer consists of two separate coils, or windings, wrapped around the same soft iron core. However, they are electrically insulated from each other.
The iron core has a substantial relative permeability. Therefore, the magnetic field lines generated due to the current in one winding are almost entirely confined within the core, such that the same magnetic flux permeates each turn of both...
The iron core has a substantial relative permeability. Therefore, the magnetic field lines generated due to the current in one winding are almost entirely confined within the core, such that the same magnetic flux permeates each turn of both...
1.8K
01:26Transformation
1.0K
Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
1.0K
01:26The Ideal Transformer
1.4K
In single-phase two-winding transformers, two windings are coiled around a magnetic core characterized by cross-sectional area A and magnetic permeability μ. A phasor current i1 enters the left winding while i2 exits the right winding, establishing the fundamental working of the transformer through electromagnetic principles.
Ampere's Law forms the basis of understanding the magnetic field within the transformer. It states that the integral of the magnetic field intensity's tangential...
Ampere's Law forms the basis of understanding the magnetic field within the transformer. It states that the integral of the magnetic field intensity's tangential...
1.4K
01:16Properties of the z-Transform II
427
The property of Accumulation in signal processing is derived by analyzing the accumulated sum of a discrete-time signal and using the time-shifting property to determine its z-transform. This principle reveals that the z-transform of the summed signal is related to the z-transform of the original signal by a multiplicative factor.
Moreover, the convolution property indicates that the convolution of two signals in the time domain corresponds to the product of their z-transforms in the frequency...
Moreover, the convolution property indicates that the convolution of two signals in the time domain corresponds to the product of their z-transforms in the frequency...
427
01:19Three-Winding Transformers
713
Three identical single-phase transformers can be configured to form a three-phase transformer connection, which involves high-voltage and low-voltage windings. The high-voltage windings are denoted by capital letters A-B-C, while the low-voltage windings are labeled with lowercase letters a-b-c, representing their respective phases. This notation helps distinguish between the high and low voltage sides of the transformer.
In the per-unit equivalent circuit of a grounded Y-Y three-phase...
In the per-unit equivalent circuit of a grounded Y-Y three-phase...
713