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Related Concept Videos

Raman Spectroscopy: Overview01:20

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
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Seeing through bone with surface-enhanced spatially offset Raman spectroscopy.

Bhavya Sharma1, Ke Ma, Matthew R Glucksberg

  • 1Department of Chemistry and ‡Deptartment of Biomedical Engineering, Northwestern University , 2145 Sheridan Road, Evanston, Illinois 60208-3113, United States.

Journal of the American Chemical Society
|November 9, 2013
PubMed
Summary
This summary is machine-generated.

Surface-enhanced spatially offset Raman spectroscopy (SESORS) can now measure through bone. This breakthrough enables non-invasive imaging of biological samples, advancing in vivo diagnostics.

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Area of Science:

  • Biophotonics
  • Spectroscopy
  • Biomedical Imaging

Background:

  • Surface-enhanced spatially offset Raman spectroscopy (SESORS) is a label-free technique with potential for in vivo imaging.
  • Previous SESORS applications were limited to subcutaneous or embedded SERS substrates.
  • In vivo imaging through bone has remained a significant challenge.

Purpose of the Study:

  • To demonstrate SESORS measurements through bone.
  • To assess the detection limits of SESORS through bone.
  • To explore quantitative analysis of through-bone SESORS data.

Main Methods:

  • Utilized SERS-active nanoparticles (nanotags) coated with Raman reporter molecules.
  • Acquired SESORS spectra through bone samples of varying thicknesses (3-8 mm).
  • Employed least-squares support vector machine analysis for quantitative detection.

Main Results:

  • Successfully acquired SESORS spectra through 3-8 mm of bone.
  • Demonstrated detection of diluted nanotag samples (~2 × 10(12) particles) through bone.
  • Validated quantitative detection capabilities using machine learning analysis.

Conclusions:

  • SESORS can effectively measure through bone, overcoming previous limitations.
  • This technique shows promise for non-invasive, real-time spectroscopic monitoring of neurochemicals through the skull.
  • Through-bone SESORS opens new avenues for biomedical applications requiring deep tissue imaging.