Related Experiment Video
Updated: May 6, 2026
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
Published on: July 7, 2020
A ternary conjugation system for the construction of DNA libraries for Geobacillus kaustophilus HTA426
Hirokazu Suzuki1, Keisuke Wada, Megumi Furukawa
1Functional Genomics of Extremophiles, Faculty of Agriculture, Graduate School, Kyushu University.
Abstract:
This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.
More Related Videos
10:41Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
Published on: January 4, 2017
11:36Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
Published on: September 23, 2017