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Characterization of polyphosphate glucokinase SCO5059 from Streptomyces coelicolor A3(2)
Mai Koide1, Akimasa Miyanaga, Fumitaka Kudo
1Department of Chemistry and Materials Science, Tokyo Institute of Technology.
Bioscience, Biotechnology, and Biochemistry
|November 9, 2013
Abstract:
SCO5059, encoded in Streptomyces coelicolor A3(2), was identified as a polyphosphate glucokinase. The Km values of SCO5059 for glucose and polyphosphate (poly(P)6) were estimated to be 12 and 4 µM, respectively, and the kcat value was 0.3 s(-1) at pH 7.7 at 28 °C. SCO5059 homologs are highly conserved among Streptomyces, and can work as polyphosphate glucokinase as well.
