Related Experiment Video
Updated: May 6, 2026

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis
Published on: June 4, 2021
Site-specific binding of a nuclear factor to the carrot extensin gene is influenced by both ethylene and wounding
1Department of Biology and Molecular Biology Institute, University of California, 90024, Los Angeles, CA, USA.
Abstract:
Experiments conducted in vitro using the electrophoretic mobility shift assay have shown that a single region of the extensin gene of carrot (Daucus carota L.) interacts with a protein factor designated Extensin Gene Binding Factor-1 (EGBF-1) present in nuclear extracts obtained from carrot roots. This interaction is sequence-specific as judged by the failure of other plant gene sequences to compete with the extensin gene for EGBF-1 binding. The EGBF-1 activity is organspecific, not being expressed in nuclear extracts obtained from carrot leaves or stems. Both ethylene treatment and wounding of roots are shown to have a controlling influence on the expression of EGBF-1 activity in nuclear extracts. These results demonstrate that at least three distinct signals: ethylene treatment, wounding, and development, are important in determining the activity of EGBF-1 in nuclear extracts, and indicate a role for EGBF-1 in stress-related signal transduction and the regulation of extensin-gene expression.
More Related Videos
08:31Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
Published on: March 29, 2019
05:22Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring
Published on: January 20, 2023
Related Concept Videos
Cell Signaling in Plants
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathways
Key Elements for Plant Nutrition
Tension Response at Adherens Junctions
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
Short-distance Transport of Resources