Related Experiment Video
Updated: May 6, 2026
Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
Published on: January 3, 2025
DNA variations in regenerated plants of pea (Pisum sativum L.)
E Cecchini1, L Natali, A Cavallini
1Department of Agricultural Plant Biology, Genetics Section, University of Pisa, Via Matteotti 1/B, I-56124, Pisa, Italy.
Abstract:
The aim of this study was to determine whether DNA variations could be detected in regenerated pea plants. Two different genotypes were analyzed by cytogenetic and molecular techniques: the "Dolce Provenza" cultivar and the "5075" experimental line. "Dolce Provenza" regenerated plants showed a reduction in DNA content, particularly at the level of unique sequences and ribosomal genes. Moreover, regeneration was associated with an increase in DNA methylation of both internal and external cytosines of the CCG sequence. On the other hand, the DNA content of the "5075" line remained stable after regeneration. DNA reduction was found only in "5075" plants regenerated from callus cultures maintained for long incubation periods (about a year). The DNA variations observed are discussed both in relation to the genotype source and the role of tissue-culture stress.
Related Concept Videos
01:18Dihybrid Crosses
01:20Monohybrid Crosses
02:27Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
02:38Asexual Reproduction
01:39Test Cross
02:26Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...