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Regulation of α-galactosidase activity and the hydrolysis of galactomannan in the endosperm of the fenugreek (Trigonella foenum-graecum L.) seed.

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D-Mannose uptake by fenugreek cotyledons.

K Zambou¹, C G Spyropoulos

¹Institute of General Botany, University of Athens, Panepistimiopolis, 15784, Athens, Greece.

|November 9, 2013

Summary

D-mannose uptake in fenugreek cotyledons involves a specific carrier, not proton-sugar cotransport. This carrier regulates D-mannose uptake capacity during seedling development.

Area of Science:

Plant Physiology
Molecular Biology
Biochemistry

Background:

D-mannose is a sugar with potential roles in plant development.
Understanding sugar uptake mechanisms is crucial for plant physiology.

Purpose of the Study:

To investigate the mechanism of D-mannose uptake in fenugreek (Trigonella foenum-graecum L.) cotyledons.
To determine if D-mannose uptake involves a carrier-mediated system or proton-sugar cotransport.

Main Methods:

Studied D-mannose uptake kinetics in detached fenugreek cotyledons.
Utilized metabolic inhibitors (carbonyl-cyanide-m-chlorophenylhydrazone, p-chloromercuribenzenesulfonic acid) to probe uptake mechanisms.
Investigated the effects of pH, competing sugars (D-galactose, 3-O-methylglucose), and turgor pressure on uptake rates.

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Main Results:

D-mannose uptake exhibited biphasic kinetics: saturable at low concentrations and diffusion-like at high concentrations.
Metabolic inhibitors significantly reduced D-mannose uptake.
D-mannose uptake was largely unaffected by pH, competing sugars, and low turgor, distinguishing it from proton-sugar cotransport.

Conclusions:

D-mannose uptake in fenugreek cotyledons is mediated by a highly specific carrier system.
This carrier plays a key role in regulating D-mannose uptake capacity during seedling development.
The uptake mechanism does not involve a proton-sugar cotransport system.