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Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo
Published on: November 7, 2013
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Mespeus--a database of metal interactions with proteins
Marjorie M Harding1, Kun-Yi Hsin
1Centre for Translational and Chemical Biology, University of Edinburgh, Edinburgh, UK.
Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2013
Summary
Mespeus is a database that visualizes experimentally determined metal-protein interactions. This resource aids in modeling and understanding the role of metals in novel protein structures and functions.
Area of Science:
- Biochemistry
- Structural Biology
- Bioinformatics
Background:
- Proteins often interact with metal ions, influencing their structure and function.
- Identifying the role of metals in novel proteins is crucial for understanding biological processes.
- Structural genomics has identified new proteins, necessitating methods to predict metal binding.
Purpose of the Study:
- To describe the Mespeus database and its utility in protein modeling.
- To demonstrate how visualizing metal-protein interactions aids in predicting metal roles in new proteins.
- To provide examples of Mespeus application in structural and functional analysis.
Main Methods:
- Utilizing the Mespeus database (http://mespeus.bch.ed.ac.uk/MESPEUS_10/) for accessing metal-protein interaction data.
- Employing modeling and analogy techniques informed by visualized interaction geometries.
- Analyzing experimentally determined metal-protein interaction data.
Main Results:
- Mespeus provides experimentally determined geometries of metal-protein interactions.
- Visualization of these interactions facilitates the modeling of metal roles in protein structure and function.
- The database serves as a valuable tool for researchers in structural genomics and proteomics.
Conclusions:
- Mespeus is a key resource for understanding metal-protein interactions.
- The database supports predictive modeling of metal ion roles in newly identified proteins.
- Visualizing metal-binding geometries enhances insights into protein function and structure.
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