Temporal control of phytochrome-dependent gene expression during radish seedling development

The level of two nuclear-encoded transcripts (the small subunit of ribulose-1,5-bisphosphate carboxylase and the chlorophyll a/b-binding protein of photosystem II) and two chloroplastencoded mRNAs (the large subunit of ribulose-1,5-bisphosphate carboxylase and the chloroplast 32-kDa polypeptide) were analysed during the first 4 d of radish (Raphanus sativus L.) seedling development. A single 5-min red-light pulse increased the concentrations of all transcripts by various orders of magnitude, depending on the particular mRNA. For all transcripts the maximal effect of the red light was observed when the irradiation was applied 48 h following sowing. The red-light treatment did not lead to changes in the total RNA level or to alterations in the levels of nuclear and chloroplastic DNAs; the level of β-actin mRNA was also unaffected by the light treatment. It is concluded that during the first days of development, cells of radish cotyledons undergo light-independent molecular changes, occurring at the transcriptional level, conferring on them a transient competence towards the photoreceptor phytochrome.