In vitro micropropagation of elite rosewood (Dalbergia latifolia Roxb.)

Induction of single and multiple shoots was obtained from nodal expiants of 60-80 year-old elite trees of rosewood on Murashige and Skoog's basal medium supplemented with 6-benzylaminopurine (1.0 mg 1(-1)) and δ -Naphthalene acetic acid (0.05 mg 1(-1)) or indole acetic acid (0.5 mg 1(-1)). Multiplication of shoots was obtained on MS (reduced major elements) or Woody Plant Medium supplemented with 6-benzylaminopurine (1.0 mg 1(-1)) and kinetin (0.5-1.0 mg 1(-1)). Excised shoots were rooted on half-strength MS with IBA (2.0 mg 1(-1)) to obtain complete plantlets. The regenerated plantlets have been acclimatized and successfully transferred to the soil.