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High-throughput tandem-microwell assay identifies inhibitors of the hydrogen sulfide signaling pathway
Yueyang Zhou1, Jing Yu, Xiling Lei
1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, 200240, China. fang.wu@sjtu.edu.cn.
Chemical Communications (Cambridge, England)
|November 12, 2013
Abstract:
We report a high-throughput assay for H2S-producing enzymes, which is based on a newly designed tandem-well plate. Screening of 21,599 agents identified several potent inhibitors of cystathionine β-synthase and cystathionine γ-lyase, the two key enzymes generating H2S in mammals, with IC50 values in the low two-digit micromolar range.
