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Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Updated: May 6, 2026

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

Exploring intercellular signaling by proteomic approaches.

¹Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada.

|November 12, 2013

Summary

This summary is machine-generated.

Proteomics technology advances enable the study of cell-cell communication, overcoming challenges in analyzing membrane and secreted proteins. This allows for a systems-level understanding of intercellular signaling in various biological contexts.

Keywords:

Intercellular signaling MS PTM Protein-protein interaction Technology

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Last Updated: May 6, 2026

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Area of Science:

Cell Biology
Proteomics
Biochemistry

Background:

Cells communicate extensively with their microenvironment through complex signaling pathways.
Understanding intercellular communication is crucial for regulating cell fate.
Current mass spectrometry (MS)-based proteomics methods face challenges in analyzing membrane and secreted proteins.

Purpose of the Study:

To review recent advancements in MS-based proteomics technology.
To highlight the application of these technologies in characterizing intercellular communication.
To discuss the systems-level profiling of intercellular signaling in diverse biological systems.

Main Methods:

Review of the latest MS-based proteomics technologies.
Application of proteomics for characterizing direct and indirect cell-cell communication.
Analysis of protein translocalization using proteomics.

Main Results:

MS-based proteomics is essential for global characterization of protein expression and post-translational modifications (PTMs).
New technologies are improving the analysis of membrane and secreted proteins involved in cell communication.
Proteomics enables systems-level profiling of intercellular signaling in contexts like tumor microenvironments and immune cell interactions.

Conclusions:

Advancements in MS-based proteomics are overcoming previous technical limitations.
Proteomics provides powerful tools for dissecting complex intercellular signaling networks.
This approach offers a systems-level view of cell communication in various physiological and pathological settings.