Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

3D printing in core facilities - Low pain, high gain.

Journal of microscopy·2026

Same author

Stress and conflict management in core facilities-News from an imaging survey.

Journal of microscopy·2026

Same author

Quantitative Mapping of Nanoscale EGFR-Grb2 Assemblies by DNA-PAINT.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026

Same author

Wash-Free Multi-Target Super-Resolution Microscopy With Photocaged DNA Labels.

Angewandte Chemie (International ed. in English)·2026

Same author

Quantitative Stimulated Emission Depletion (STED) Microscopy with DNA-Fluorophore Labels.

ACS nano·2026

Same author

Fast, Bright, and Reversible Fluorescent Labeling of Rhodamine-Binding Proteins.

Journal of the American Chemical Society·2025

Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026

Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026

Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026

Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026

Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026

Same journal

<i>In vivo</i> RNA targeting in the nematode <i>Caenorhabditis elegans</i> using exogenous catalytic DNA.

BioTechniques·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 6, 2026

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

Correlative light microscopy for high-content screening.

Benjamin Flottmann¹, Manuel Gunkel, Tautvydas Lisauskas

¹BioQuant Centre, Heidelberg University, Heidelberg, Germany.

|November 13, 2013

Summary

This summary is machine-generated.

This study introduces correlative light microscopy, combining widefield, confocal, and super-resolution imaging. This approach enhances spatial resolution for scalable biological experiments, enabling detailed cell analysis.

More Related Videos

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Published on: November 29, 2018

Cellular Redox Profiling Using High-content Microscopy

Cellular Redox Profiling Using High-content Microscopy

Published on: May 14, 2017

Related Experiment Videos

Last Updated: May 6, 2026

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Published on: November 29, 2018

Cellular Redox Profiling Using High-content Microscopy

Cellular Redox Profiling Using High-content Microscopy

Published on: May 14, 2017

Area of Science:

Cell Biology
Microscopy Techniques
Biotechnology

Background:

High-throughput microscopy offers rapid data collection but suffers from low spatial resolution.
Bridging the gap between speed and detail is crucial for advancing biological research.

Purpose of the Study:

To develop and demonstrate a correlative light microscopy workflow combining different imaging modalities.
To enable scalable experiments with improved spatial resolution for cell biology studies.

Main Methods:

Integration of automated widefield imaging for initial cell selection.
Precise re-localization of selected cells using micrometer precision.
Subsequent high-resolution imaging via confocal and super-resolution microscopy.

Main Results:

Successful visualization and quantification of cis- and trans-Golgi markers.
Demonstration of a scalable workflow for correlative imaging.
Validation of the approach for analyzing cellular structures at increasing resolutions.

Conclusions:

Correlative light microscopy effectively combines high-throughput screening with high-resolution imaging.
This integrated approach significantly enhances the potential for scalable and detailed biological investigations.
The workflow provides a robust method for precise cellular structure analysis.