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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
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Related Experiment Video

Updated: May 6, 2026

Measuring the 50% Haemolytic Complement CH50 Activity of Serum
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Evaluation of complement function by ELISA.

Anja Roos1, Jörgen Wieslander

  • 1Wieslab B.V., Eurodiagnostica, Nijmegen, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|November 13, 2013
PubMed
Summary
This summary is machine-generated.

ELISA assays are replacing traditional hemolytic assays for evaluating human complement system function. This advancement enables better standardization and analysis of classical, alternative, and lectin pathways in diagnostics.

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Area of Science:

  • Immunology
  • Clinical Diagnostics
  • Biochemistry

Background:

  • Complement system evaluation is crucial for human serum diagnostics.
  • Traditional hemolytic assays (CH50, AP50) are being superseded by ELISA.
  • ELISA offers improved standardization and broader pathway analysis.

Purpose of the Study:

  • To describe ELISA methodologies for complement pathway assessment.
  • To detail the functional analysis of classical, alternative, and lectin pathways.
  • To highlight ELISA's role in clinical laboratory diagnostics.

Main Methods:

  • Enzyme-Linked Immunosorbent Assays (ELISA) for complement function.
  • Assessment of classical pathway activity.
  • Assessment of alternative pathway activity.
  • Assessment of MBL-dependent lectin pathway activity.
  • Assessment of Ficolin-3-dependent lectin pathway activity.

Main Results:

  • ELISA assays provide a standardized method for complement pathway evaluation.
  • ELISA facilitates functional analysis of multiple complement pathways.
  • This methodology is applicable to clinical laboratory diagnostics.

Conclusions:

  • ELISA assays represent a significant advancement in complement diagnostics.
  • The described ELISA methods allow comprehensive assessment of complement system function.
  • These assays enhance diagnostic capabilities for complement-related disorders.