Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Complement System

Complement System

The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Three cryo-EM structures of complement C3d-bound α<sub>M</sub>β<sub>2</sub> reveal an unexpected layer of dynamics for αI-containing integrin receptors.

Science advances·2026

Same author

Structure-function analysis of empasiprubart, a calcium- and pH-dependent clinical phase complement C2 blocking antibody.

mAbs·2026

Same author

Nanobody-based IgG simultaneously inhibit the allergenic and enzymatic activity of the dominant honeybee venom allergen.

Nature communications·2026

Same author

Antagonist nanobodies prevent protease inhibition by CD109.

The Journal of biological chemistry·2026

Same author

Nanobody-based gene therapy targeting complement component C3 reduces choroidal neovascularization in mice.

Molecular therapy. Methods & clinical development·2025

Same author

Randomized phase I trial outcomes show safe and sustainable inhibition of classical and lectin complement pathways by empasiprubart.

Nature communications·2025

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 6, 2026

Measuring the 50% Haemolytic Complement CH50 Activity of Serum

Measuring the 50% Haemolytic Complement CH50 Activity of Serum

Published on: March 29, 2010

Purification of human complement protein C5.

Lars Sottrup-Jensen¹, Gregers Rom Andersen

¹Department of Molecular Biology and Genetics, University of Aarhus, Aarhus C, Denmark.

Methods in Molecular Biology (Clifton, N.J.)

|November 13, 2013

Summary

This summary is machine-generated.

Researchers developed a new method to purify complement C5, a key protein in immune responses. This streamlined process yields homogenous, crystallizable C5, overcoming previous purification challenges.

More Related Videos

Depletion of Specific Cell Populations by Complement Depletion

Depletion of Specific Cell Populations by Complement Depletion

Published on: February 5, 2010

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Published on: July 20, 2016

Related Experiment Videos

Last Updated: May 6, 2026

Measuring the 50% Haemolytic Complement CH50 Activity of Serum

Measuring the 50% Haemolytic Complement CH50 Activity of Serum

Published on: March 29, 2010

Depletion of Specific Cell Populations by Complement Depletion

Depletion of Specific Cell Populations by Complement Depletion

Published on: February 5, 2010

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Published on: July 20, 2016

Area of Science:

Immunology
Protein Biochemistry

Background:

Complement C5 is crucial for immune responses, generating C5a and C5b during complement activation.
Purifying C5 from plasma is challenging due to low abundance and overlap with C3 during chromatography.

Purpose of the Study:

To describe a novel procedure for purifying complement C5.
To obtain homogenous, monodisperse, and crystallizable C5.

Main Methods:

Chromatographic separation techniques were optimized.
Specific protocols were developed to address C5's low concentration and C3 interference.

Main Results:

A robust purification procedure for complement C5 was established.
The method yields homogenous, monodisperse, and crystallizable C5 protein.

Conclusions:

This new method simplifies C5 purification, enabling further structural and functional studies.
The availability of purified C5 will advance research in complement biology and related diseases.