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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 6, 2026

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry

Published on: May 19, 2020

Detection of CD59 using flow cytometry.

¹Division of Transplantation Immunology and Mucosal Biology, MRC Centre for Transplantation, King's College London, Guy's Hospital, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 13, 2013

Summary

This summary is machine-generated.

CD59 regulates the complement system and protects cells from damage. This study introduces a new method to analyze CD59 expression on cell surfaces, aiding research into diseases like Paroxysmal nocturnal hemoglobinuria (PMH) and cancer.

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Last Updated: May 6, 2026

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Published on: May 19, 2020

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Area of Science:

Immunology
Cell Biology

Background:

CD59 is the sole regulator of the terminal complement pathway.
CD59 expression protects cells from complement-mediated lysis.
Dysregulation of CD59 is linked to Paroxysmal nocturnal hemoglobinuria (PMH) and various cancers.

Purpose of the Study:

To develop and present a novel method for assessing CD59 expression.
To facilitate the study of CD59's role in cellular protection and disease pathogenesis.

Main Methods:

The study details a specific methodology for quantifying CD59.
This method focuses on detecting CD59 on the cell surface.

Main Results:

A reliable method for studying CD59 surface expression has been established.
This technique enables further investigation into CD59's biological functions.

Conclusions:

The described method provides a valuable tool for researchers.
It will advance the understanding of CD59's involvement in health and disease states.