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Related Experiment Video

Updated: May 6, 2026

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Published on: December 17, 2013

Functional analysis of CD59 using complement-dependent cytotoxicity assay.

¹Division of Transplantation Immunology and Mucosal Biology, MRC Centre for Transplantation, King's College London, Guy's Hospital, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 13, 2013

Summary

This summary is machine-generated.

CD59 overexpression protects tumors from complement lysis, a key mechanism of antibody therapy. This study presents a method to assess this CD59-mediated tumor protection, aiding in the development of more effective cancer treatments.

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Last Updated: May 6, 2026

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

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Published on: December 17, 2013

High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds

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Real-time Cytotoxicity Assays in Human Whole Blood

Real-time Cytotoxicity Assays in Human Whole Blood

Published on: November 7, 2014

Area of Science:

Immunology
Oncology
Molecular Biology

Background:

Tumor resistance to therapeutic anticancer antibodies is a significant clinical challenge.
Complement lysis, mediated by the complement system, is a critical effector function of many anticancer antibodies.
CD59 is a known inhibitor of complement activation and has been implicated in tumor immune evasion.

Purpose of the Study:

To provide a method for quantifying the protective effect of CD59 against complement-mediated lysis in tumors.
To enable researchers to study the role of CD59 in tumor resistance to antibody therapy.

Main Methods:

The chapter details a specific experimental approach to assess CD59-mediated complement resistance.
This method allows for the evaluation of tumor cell susceptibility to complement-dependent cytotoxicity (CDC).

Main Results:

The described method allows for the measurement of tumor protection conferred by CD59.
This facilitates a deeper understanding of the mechanisms underlying therapeutic antibody resistance.

Conclusions:

CD59 plays a crucial role in protecting tumors from complement-dependent killing by therapeutic antibodies.
The presented method is valuable for evaluating CD59's contribution to therapeutic resistance and for guiding treatment strategies.