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Complement System

Complement System

The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...

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Updated: May 6, 2026

Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

Published on: June 17, 2020

Analysis of the complement activation in mice.

Nirmal K Banda¹, Kazue Takahashi

¹Pediatrics, Massachusetts General Hospital, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 13, 2013

Summary

This summary is machine-generated.

This study details methods to assay the three complement pathways (classical, lectin, alternative) in mice. Researchers measure deposited cleaved complement proteins to understand this crucial immune mechanism.

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Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

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Published on: January 29, 2014

Area of Science:

Immunology
Biochemistry

Background:

Complement activation is a vital immune process in both mice and humans.
The murine complement system comprises three distinct activation pathways: classical, lectin, and alternative.

Purpose of the Study:

To describe methodologies for assaying the three complement pathways in a murine model.
To provide a framework for analyzing complement activation in mice.

Main Methods:

Assaying complement pathway activation by measuring deposited cleaved complement proteins.
Utilizing materials known to activate specific complement pathways for detection.

Main Results:

The described methods allow for the quantitative assessment of deposited cleaved complement proteins.
Successful measurement of deposited complement proteins indicates pathway activation.

Conclusions:

Established methods enable the assay of classical, lectin, and alternative complement pathways in mice.
Measuring deposited cleaved complement proteins is a reliable technique for assessing murine complement activation.