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Related Experiment Video

Updated: May 6, 2026

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens
08:47

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Published on: April 5, 2019

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Opsonophagocytic assay.

Markryan Dwyer1, Mihaela Gadjeva

  • 1Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 13, 2013
PubMed
Summary
This summary is machine-generated.

The opsonophagocytic killing (OPK) assay measures how well antibodies from vaccine candidates work. This study presents two protocols for the OPK assay using human neutrophils or HL-60 cells to assess vaccine efficacy.

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Area of Science:

  • Immunology
  • Vaccinology
  • Microbiology

Background:

  • The opsonophagocytic killing (OPK) assay is crucial for evaluating vaccine-induced antibody function.
  • It serves as a correlate for protection, assessing the capacity of antibodies to mediate pathogen clearance.

Purpose of the Study:

  • To describe standardized protocols for performing the OPK assay.
  • To enable the assessment of functional vaccine-induced antibodies against various pathogens.

Main Methods:

  • Detailed protocols for an in vitro OPK assay are presented.
  • Assays utilize either primary human polymorphonuclear neutrophils (PMNs) or the cultured HL-60 cell line.

Main Results:

  • The study provides reproducible methods for OPK assay implementation.
  • Both human PMNs and HL-60 cells can be effectively used in the described OPK protocols.

Conclusions:

  • Standardized OPK assays are vital for vaccine development and selection.
  • These protocols facilitate the measurement of functional antibody responses, aiding in the identification of promising vaccine candidates.