Tumor-suppressing effects of miR451 in human osteosarcoma
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Updated: May 6, 2026
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
Published on: October 28, 2021
Haidong Xu1, Qiang Mei, Luxia Shi
1Department of Orthopedics of Jinling Hospital, Nanjing University, School of Medicine, 305 Zhongshan East Road, Nanjing, 210002, China.
Abstract:
Osteosarcoma is the 3rd most common human cancer in childhood and young adults, and is the leading cause of mortality. Recent studies suggest that miRNAs could regulate the growth and progression of osteosarcoma, indicating some novel targets for therapy. In our study, we demonstrated that miR-451 was down-regulated in human osteosarcoma U2OS, SAOS, and MG63 cells lines as well as in tumor tissue surgically resected compared with the normal tissues. Overexpression of miR-451 inhibited cell proliferation and resulted in cell apoptosis in osteosarcoma cells. G1 cell cycle arrest was also induced by miR-451. Repressed by miR-451, PGE2 and CCND1 reversed the inhibitory effects of miR-451 on proliferation. In conclusion, miR-451 played a tumor-suppressing role through modulating the expression of PGE2 and CCND1, suggesting a novel target for the diagnosis and treatment of osteosarcoma.
Insights
MicroRNA-451 (miR-451) acts as a tumor suppressor in osteosarcoma. Its downregulation promotes cancer growth, while its restoration inhibits proliferation and induces apoptosis, offering a potential therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Osteosarcoma is a prevalent and deadly cancer in young individuals.
- MicroRNAs (miRNAs) are increasingly recognized for their role in cancer regulation.
- Identifying specific miRNAs involved in osteosarcoma progression is crucial for therapeutic development.
Purpose of the Study:
- To investigate the role of miR-451 in osteosarcoma.
- To determine the effect of miR-451 expression on osteosarcoma cell behavior.
- To identify potential downstream targets regulated by miR-451.
Main Methods:
- Quantification of miR-451 levels in osteosarcoma cell lines (U2OS, SAOS, MG63) and patient tumor tissues.
- Overexpression of miR-451 in osteosarcoma cells.
- Assessment of cell proliferation, apoptosis, and cell cycle progression.
- Analysis of prostaglandin E2 (PGE2) and cyclin D1 (CCND1) expression.
Main Results:
- miR-451 was significantly downregulated in osteosarcoma cells and tissues.
- Overexpression of miR-451 inhibited osteosarcoma cell proliferation and induced apoptosis.
- miR-451 induced G1 cell cycle arrest.
- PGE2 and CCND1 partially reversed the inhibitory effects of miR-451.
Conclusions:
- miR-451 functions as a tumor suppressor in osteosarcoma.
- miR-451 exerts its effects by modulating PGE2 and CCND1 expression.
- miR-451 represents a potential diagnostic and therapeutic target for osteosarcoma.
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