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Enhanced accuracy of single-molecule diffusion measurements with a photocleavable energy-transfer dyad
Maren Dill1, Moritz C Baier, Stefan Mecking
1Fachbereich Chemie, Universität Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany).
Angewandte Chemie (International Ed. in English)
|November 14, 2013
Abstract:
A photocleavable energy-transfer dyad was synthesized, characterized, and applied to single-molecule fluorescence microscopy. After photocleavage, a combination of independent two-color single-molecule tracking and analysis of single-molecule energy-transfer efficiencies allows the determination of the temporal evolution of the relative distances between both fragments from the nm to the μm scale. This gives access to a broad range of diffusion coefficients.

