Related Experiment Video

Updated: May 6, 2026

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping
06:22

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping

Published on: October 27, 2020

4.4K

Intrinsic features in microRNA transcriptomes link porcine visceral rather than subcutaneous adipose tissues to

Jideng Ma1, Zhi Jiang, Shen He

  • 1Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.

Plos One
|November 14, 2013
PubMed

Insights

This study reveals distinct microRNA (miRNA) profiles in visceral adipose tissues (VATs) versus subcutaneous adipose tissues (SATs). VAT-specific miRNAs are linked to immune and inflammation responses, suggesting a role in metabolic disorder risk.

Area of Science:

  • Molecular Biology
  • Genomics
  • Metabolomics

Background:

  • MicroRNAs (miRNAs) are key post-transcriptional regulators of gene expression.
  • Visceral adipose tissues (VATs) and subcutaneous adipose tissues (SATs) exhibit distinct characteristics.
  • Limited research exists on miRNA transcriptome differences between VATs and SATs.

Purpose of the Study:

  • To comprehensively investigate and compare miRNA transcriptomes between porcine VATs and SATs.
  • To identify differentially expressed miRNAs and their potential roles in adipose tissue function and metabolic risk.

Main Methods:

  • Small RNA sequencing was employed across six porcine adipose tissue variants.
  • miRNA identification, quantification, and comparative analysis were performed.
  • miRNA target prediction was utilized to infer functional associations.

Main Results:

  • 219 known miRNAs, 97 novel miRNA*s, and 124 conserved miRNAs were identified.
  • Universally abundant miRNAs (e.g., miR-148a-3p, miR-143-3p) suggest housekeeping roles in adipogenesis.
  • Significant miRNA expression variations were observed between VATs and SATs, with VATs enriched in immune/inflammation-related miRNAs.

Conclusions:

  • Distinct miRNA expression patterns exist between VATs and SATs, reflecting their intrinsic differences.
  • VAT-specific miRNAs are predominantly associated with immune and inflammation responses.
  • These findings suggest VATs may be closely linked to an increased risk of metabolic disorders.

More Related Videos

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function09:20

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

3.4K
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues09:20

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Published on: March 31, 2016

30.7K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.2K
MicroRNAs01:22

MicroRNAs

9.8K
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
2.1K