Related Experiment Video
Updated: May 6, 2026
The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration
Published on: November 26, 2015
The astroglial reaction along the mouse cochlear nerve following inner ear damage
Zhengqing Hu1, Baofu Zhang, Xuemei Luo
1Department of Otolaryngology-HNS, Wayne State University School of Medicine, Detroit, Michigan, USA.
Objective:
Determine how the astroglial cells of the peripheral and central nervous system transitional zone (PCTZ) react to sensorineural hearing loss using a mouse cochlear nerve model.
Study Design:
Prospective, basic science.
Setting:
Research laboratory.
Subjects And Methods:
Neomycin was injected into the mouse inner ear to cause chemically induced hearing loss. Auditory brainstem responses (ABRs) were used to determine hearing threshold shifts after neomycin treatment. Immunofluorescence was used to detect the expression of proteins specific for hair cells, spiral ganglion neurons, astrocytes, and the myelin components of both oligodendrocytes and Schwann cells.
Results:
ABR threshold shifts and immunofluorescence results supported that hair cells and spiral ganglion neurons were damaged in neomycin-treated mice. Immunofluorescence showed the peripheral and central nervous system (PNS and CNS) transitional zone of the cochlear nerve at the interface of the myelin components of the PNS and CNS. In the control mice, the expression of glial fibrillary acidic protein (GFAP) was observed proximally to the PCTZ closer to the CNS, which is their normal location. However, in neomycin-treated animals the expression of GFAP was detected distally to the PCTZ and was found close to the spiral lamina level in the basal cochlear turn, suggesting that GFAP-expressing astrocytes migrated across the PCTZ and reached the PNS.
Conclusion:
The GFAP positive astrocyte processes extended across the PCTZ along the mouse cochlear nerve following chemically induced sensorineural hearing loss.
