Back to basics: histological, microbiological and biochemical sampling in wound care

Despite the advances in our understanding of normal and abnormal wound healing over the past decade, a specific biomarker which can be used clinically to identify 'hard-to-heal' wounds still remains elusive. Tissue and exudate samples extracted from the site of the wound are traditionally analysed using histological, microbiological and biochemical laboratory techniques. The aim of this paper is to review the advantages of these techniques, their limitations and the rapid developments in the fields of microbiology and proteomics, which are offering new insights into the differences between healing and non-healing wounds.