Related Experiment Video
Updated: May 6, 2026

08:35
In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
Published on: June 12, 2017
10.0K
Autophagy during beef aging.
Marina García-Macia1, Verónica Sierra2, Ana Palanca1
1Departamento de Morfología y Biología Celular; Facultad de Medicina; Universidad de Oviedo; Oviedo, Spain.
Autophagy
|November 15, 2013
Summary
Autophagic processes were observed in beef muscle postmortem, impacting meat quality. Differences between Asturiana de los Valles and Asturiana de la Montaña breeds suggest a role in meat maturation.
Area of Science:
- Meat Science
- Cellular Biology
- Animal Science
Background:
- Muscle conversion to meat is critical for meat quality.
- Autophagy, a cellular degradation process, is key to understanding postmortem changes.
- Investigating autophagy in different beef breeds can reveal breed-specific maturation mechanisms.
Purpose of the Study:
- To investigate the occurrence and role of autophagic processes during early postmortem muscle conversion in beef.
- To compare the timing and characteristics of autophagic activity between two distinct beef breeds.
Main Methods:
- Analysis of muscle tissue from Asturiana de los Valles and Asturiana de la Montaña beef breeds.
- Examination of muscle tissue during the early postmortem period (2 to 24 hours).
Main Results:
- Autophagic processes were detected in beef muscle tissue within the first 24 hours postmortem.
- Significant time-scale differences in autophagic activity were observed between the two breeds.
- These findings suggest a role for autophagy in the meat maturation process.
Conclusions:
- Autophagy occurs during early postmortem beef muscle conversion.
- Breed-specific differences in autophagic timing may influence meat maturation.
- This study provides novel insights into the cellular mechanisms governing meat quality.
Related Concept Videos
Autophagy
5.1K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.1K
Delivery Pathways to the Lysosome
7.6K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
7.6K
Autophagic Cell Death
3.3K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.3K
Cellular Injury V: Apoptosis and Autophagy
121
Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...
121
Bacterial Protein Maturation
748
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
748
Phagocytosis of Apoptotic Cells
3.9K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
3.9K

